Challenges and progress related to gene editing in rare skin diseases.

Genodermatoses represent a large group of inherited skin disorders encompassing clinically-heterogeneous conditions that manifest in the skin and other organs. Depending on disease variant, associated clinical manifestations and secondary complications can severely impact patients' quality of life and currently available treatments are transient and not curative. Multiple emerging approaches using CRISPR-based technologies offer promising prospects for therapy. Here, we explore current advances and challenges related to gene editing in rare skin diseases, including different strategies tailored to mutation type and structural organization of the affected gene, considerations for in vivo and ex vivo applications, the critical issue of delivery into the skin, and immune aspects of therapy. Against the backdrop of a landmark FDA approval for the first re-dosable gene replacement therapy for a rare genetic skin disorder, gene editing approaches are inching closer to the clinics and the possibility of a local permanent cure for patients affected by these disorders.

Toxicity test profile for deep eutectic solvents: A detailed review and future prospects.

Deep eutectic solvents (DESs) are preferable in terms of starting materials, storage and synthesis, simplicity, and component material affordability. In several industries ranging from chemical, electrochemical, biological, biotechnology, material science, etc., DES has demonstrated remarkable potential. Despite all these accomplishments, the safety issue with DES must be adequately addressed. Different DES interacts with the cellular membranes differently. It is not possible to classify all DES as easily biodegradable. By expanding the current understanding of the toxicity and biodegradation of DES, interactions between organisms and cellular membranes can be linked. The DES toxicity profile varies according to their concentration, the nature of the individual components, and how they interact with living things. Therefore, the results of this review can serve as a baseline for DES development in the future.

Meta-QTL analysis in wheat: progress, challenges and opportunities.

Wheat, an important cereal crop globally, faces major challenges due to increasing global population and changing climates. The production and productivity are challenged by several biotic and abiotic stresses. There is also a pressing demand to enhance grain yield and quality/nutrition to ensure global food and nutritional security. To address these multifaceted concerns, researchers have conducted numerous meta-QTL (MQTL) studies in wheat, resulting in the identification of candidate genes that govern these complex quantitative traits. MQTL analysis has successfully unraveled the complex genetic architecture of polygenic quantitative traits in wheat. Candidate genes associated with stress adaptation have been pinpointed for abiotic and biotic traits, facilitating targeted breeding efforts to enhance stress tolerance. Furthermore, high-confidence candidate genes (CGs) and flanking markers to MQTLs will help in marker-assisted breeding programs aimed at enhancing stress tolerance, yield, quality and nutrition. Functional analysis of these CGs can enhance our understanding of intricate trait-related genetics. The discovery of orthologous MQTLs shared between wheat and other crops sheds light on common evolutionary pathways governing these traits. Breeders can leverage the most promising MQTLs and CGs associated with multiple traits to develop superior next-generation wheat cultivars with improved trait performance. This review provides a comprehensive overview of MQTL analysis in wheat, highlighting progress, challenges, validation methods and future opportunities in wheat genetics and breeding, contributing to global food security and sustainable agriculture.

Th17-associated cytokines IL-17 and IL-23 in inflamed skin of Darier disease patients as potential therapeutic targets.

Darier disease (DD) is a rare, inherited multi-organ disorder associated with mutations in the ATP2A2 gene. DD patients often have skin involvement characterized by malodorous, inflamed skin and recurrent, severe infections. Therapeutic options are limited and inadequate for the long-term management of this chronic disease. The aim of this study was to characterize the cutaneous immune infiltrate in DD skin lesions in detail and to identify new therapeutic targets. Using gene and protein expression profiling assays including scRNA sequencing, we demonstrate enhanced expression of Th17-related genes and cytokines and increased numbers of Th17 cells in six DD patients. We provide evidence that targeting the IL-17/IL-23 axis in a case series of three DD patients with monoclonal antibodies is efficacious with significant clinical improvement. As DD is a chronic, relapsing disease, our findings might pave the way toward additional options for the long-term management of skin inflammation in patients with DD.

Unleashing the visible light-exposed photocatalytic potential of V2O5/g-C3N4 nanocomposites for dye industries wastewater cleaner production.

Dealing harmful dye-containing effluent from the textile sector significantly contributes to water contamination. The persistence of these dyes in wastewater complicates traditional treatment approaches, emphasizing the necessity for efficient photocatalytic materials for dye pollution degradation. Due to its unique features, V2O5/g-C3N4 nanocomposites are discovered as promising photocatalysts in this area. The V205 nanoparticles act as electron acceptors, while g-C3N4 acts as electron donors, thus encouraging charge separation and increasing photocatalytic activity. The V2O5/g-C3N4 nanocomposites are characterized using XRD, FTIR spectroscopy, SEM, TEM, XPS, and UV-DRS. Cationic dyes, anionic dyes and mix dyes (1:1 mixture of cationic and anionic dyes) are used to test the photocatalytic activity of the nanocomposites. Photocatalytic activity shows that V2O5/g-C3N4 nanocomposites are more active than their precursors. The V5G-2 nanocomposite degrades anionic (Rose Bengal (85.1%) and Xylenol Orange (77.6%), cationic (Auramine O (75% and Crystal Violet (79.5%), and mixed dyes (81%), after 120 min of irradiation. This study introduces a novel technique for synthesizing V2O5/g-C3N4 nanocomposites using solvothermal and ultrasonic processes. The findings of this research provide significant knowledge for the development of photocatalysts with enhanced efficiency in the degradation of dye pollutants.

Eco-friendly phytofabrication of Ficus Benjamina L. based ZnO-doped g-C3N4 nanocomposites for remarkable photocatalysis and antibacterial applications.

The research reported here emphasizes the phytoextract route synthesized ZnO-doped g-C3N4 (GCN) for its photocatalytic activity, which helps to ensure a sustained & healthy environment. The leaf extract solution of Ficus Benjamina L. was used for the synthesis of ZnO nanoparticles, and GCN was prepared via urea using a thermal polymerization process. The flower extract functions as both stabilizers and capping agents during the process of synthesis of ZnO nanoparticles. The synthesized nanocomposites were then calcined at 400 °C and were further characterized with spectroscopy (UV-Vis), diffracted pattern (XRD), and infrared spectroscopy (FTIR). Further, the photocatalytic activity of auramine orange (AO) and methylene blue (MB) dye from phytoextract route synthesized pure ZnO NPs, GCN-Pure, and composites with varied millimolar concentrations of ZnO nanoparticles with GCN of the constant amount was checked. After the complete analysis, it was observed that the series that was prepared of ZnO-GCN nanocomposites showed notable enhancement in the degradation pattern of the methylene blue dye. Apparently, 1.5 mmol (mM) ZnO-GCN presented greater degradation patterns for Auramine orange and Methylene blue dye as compared to other nanocomposites that were synthesized. The observed increased photocatalytic activity has a conceivable explanation. The antibacterial activity studies of the prepared nanocomposites were also performed against the E. coli strain showing an enhanced zone of inhibition towards it.

Tea (Camellia sinensis (L.) Kuntze) as an emerging source of protein and bioactive peptides: A narrative review.

Tea residues represent one of the major agricultural wastes that are generated after the processing of tea. They account for 21-28% of crude protein and are often discarded without the extraction of valuable proteins. Due to various bioactivity and functional properties, tea proteins are an excellent alternative to other plant-based proteins for usage as food supplements at a higher dosage. Moreover, their good gelation capacity is ideal for the manufacturing of dairy products, jellies, condensation protein, gelatin gel, bread, etc. The current study is the first to comprehend various tea protein extraction methods and their amino acid profile. The preparation of tea protein bioactive peptides and hydrolysates are summarized. Several functional properties (solubility, foaming capacity, emulsification, water/oil absorption capacity) and bioactivities (antioxidant, antihypertensive, antidiabetic) of tea proteins are emphasized.

A Comparative Study Between Plate Osteosynthesis and Intramedullary Nailing for Diaphyseal Fracture of Radius and Ulna in Adults.

INTRODUCTION: In this era of active living, industrial growth, increasing automobile accidents, and athletic activities, fractures of the forearm bones are becoming more frequent. The incidence of diaphyseal fractures of both bone forearms is reported to be approximately 10 per 10,000 persons per year, although rates may vary according to age and sex. If not properly treated, a fracture of the forearm bones might cause a serious loss of function. Therefore, to restore function, these fractures require adequate anatomical reduction and internal fixation. The majority of forearm fractures in adults are treated surgically, and various modes of internal fixation are available. In this study, we evaluated and compared the clinical, functional, and radiological outcomes of both bone forearm diaphyseal fractures treated with plate osteosynthesis and intramedullary nailing. MATERIAL AND METHOD: This prospective and comparative study was conducted in a tertiary care medical teaching hospital in southern Rajasthan, India. Forty patients with diaphyseal fractures of the radius and ulna bones who presented to the casualty or orthopedic outpatient departments of our institute were included. Patients were divided into two groups, 20 patients in each group and treated by intramedullary nailing (group A) and plate osteosynthesis (group B), and regularly followed up and evaluated for clinical, functional, and radiological outcomes. RESULT: Final results were calculated according to the modified Grace-Eversmann scoring system. In group A, out of 20 cases excellent score was seen in six cases (30%), good score in nine cases (45%), fair score in three cases (15%), and at last, two cases (10%) showed poor results. In group B, excellent score was in eight cases (40%), good score was in eight cases (40%), fair score was in three cases (15%), and at last, one case (5%) was poor in our study. CONCLUSION: Based on our findings, we conclude that for the treatment of diaphyseal fractures of the radius and ulna, both treatment modalities provide equally satisfactory results.

Surveillance of Antimicrobial Susceptibility Pattern of Chronic Suppurative Otitis Media in Tertiary Care Hospital of Southern Rajasthan.

The main aim of this work is to identify aerobic bacteriology and antibiotic sensitivity pattern of chronic suppurative otitis media in tertiary care hospital of southern Rajasthan. Two hundred and fifty clinically diagnosed cases of chronic suppurative otitis media of all age groups and both the sexes with the ear discharged of more than 6 weeks duration formed the subject of the study group. The specific identification of bacterial pathogens is done based on microscopic morphology, staining characteristics, cultural and biochemical properties using standard laboratory procedures. Antimicrobial susceptibility of the bacterial isolates to the commonly used antibiotics according to CLSI guideline is done by Kirby-Bauer disc diffusion method. Out of 250 cases, 226 (90.4%) were found to be smear positive and culture positive, 17 (6.8%) were found smear positive and culture negative, 7 (2.8%) were smear negative and culture negative. Pseudomonas spp was found the most common organism isolated was. Out of 244 isolates, 174 (71.3%) were found to be sensitive to Amikacin. In our study, Pseudomonas spp. 98% isolates were found to be most sensitive to Meropenem and 84.2% isolates were found to be most resistance to Ceftazidime. This study is useful to prevents the administration of unwanted antibiotics and in development of empirical policy. This may be helpful to medical practitioner for the prescribing antibiotics in the treatment of CSOM.

A Prospective Study on Functional Outcomes of Serial Cast Correction in Congenital Talipes Equinovarus (CTEV) by Ponseti Method.

INTRODUCTION: Congenital talipes equinovarus (CTEV), often known as clubfoot, is one of the most common congenital deformities of the foot and ankle, affecting one in every 1,000 live births. The deformity has four components: ankle equinus, hindfoot varus, forefoot adductus, and midfoot cavus. The Ponseti casting technique of CTEV management has proven to be more effective, generating higher positive outcomes and lesser complications than conventional surgical methods. METHODS: This study was conducted in a tertiary care teaching hospital centre in southern Rajasthan, India. Twenty cases with 31 feet of untreated clubfoot were included. The deformity was scored according to Pirani's scoring system. Manipulation and serial corrective casts were applied at weekly intervals according to Ponseti's method. Percutaneous tendoachilles tenotomy was done whenever required (guided by Pirani score). Final results were calculated using the Pirani score, noted before putting the patient on foot abduction orthosis. RESULTS: In our study, the average number of casts required for full correction was 6.5. Heel cord tenotomy was required in 27 feet (87%) to achieve full correction. Final Pirani score significantly improved from an average of 4.8 on presentation to 0.055 after completion of casting. CONCLUSION: We conclude that the Ponseti technique of CTEV correction significantly reduces the need for invasive surgical procedures along with being exceedingly safe, effective, and affordable. The Ponseti technique of cast correction is crucial and provides a painless, plantigrade, cosmetically acceptable foot with higher functional outcomes and minimal complications.

Bridge Plating Versus Hybrid External Fixation in the Management of Proximal Tibia Metaphyseal Fractures.

BACKGROUND: Due to the surge in high-speed road traffic accidents during the past few years, extra-articular proximal tibia fractures have also risen in number. For the treatment of such fractures, various options are available like conservative treatment with casting, surgical treatment with plate osteosynthesis, or a hybrid external fixator. Exposure of the bone surface and extensive soft tissue dissection is needed in bridge plating, posing a risk of bleeding, infection, and soft-tissue healing issues, and the blood supply of the fractured area is also hampered as the periosteum is also destroyed. To avoid these complications, a hybrid external fixator can be used but it has its own risks of malunion, non-union, and pin-tract infections; another drawback is patient compliance. In this study, we compare the clinical and functional outcomes of two treatment modalities, i.e., bridge plating and hybrid external fixator, in the treatment of proximal tibia metaphyseal fractures. MATERIALS AND METHODS: This prospective randomized study was conducted from February 2021 to June 2022 on 46 adult patients diagnosed with proximal tibia metaphyseal fracture and willing to participate. An odd number of patients were treated with a bridge plate and even with a hybrid external fixator. RESULTS: A total of 46 patients were included in the study, sustaining proximal tibia metaphyseal fracture, out of which 23 patients underwent hybrid external fixation with an outcome of 69.43 ± 8.11 according to the Knee Society Score (KSS) and 23 for bridge plating which showed better results as their score at final follow-up was 75.00 ± 8.22. CONCLUSION: According to our study, we found that bridge plating is a better treatment modality than the hybrid external fixator as the former showed better postoperative knee range of motion and functional outcome and led to a smaller number of complications. But the clinical outcome would also be influenced by the type of fracture, degree of comminution, type of injury, i.e., open or closed, and quality of bone.

Clinical and Functional Outcomes of Anatomical Plating in Distal Humerus Fractures in Adults.

Background Overall, 2% of all adult fractures are attributed to fractures of the distal humerus, including both supracondylar and intercondylar fractures. According to recent studies, stable fixation with anatomical reduction of intra-articular fragments and early mobilization is required for best outcomes. This study included patients with distal end humerus fractures treated by open reduction and internal fixation (ORIF) with anatomical locking plates and evaluated their clinical outcomes. Methodology This prospective study was conducted at a medical college teaching hospital in southern Rajasthan, India. In total, 20 adult patients with distal end humerus fractures who presented to the orthopedic outpatient department or casualty were admitted. Patients were treated by ORIF with anatomical locking plates, followed up, and evaluated for clinical and functional outcomes. Results Using the Mayo Elbow Performance Score, out of 20 cases, excellent results were noted in five patients, good results in seven patients, fair results in six patients, and poor results in two patients. Conclusions Locking plates are reliable and effective management options for distal humerus fractures. As locking plates are strong and rigid, the period of immobilization can be decreased. Early mobilization helps in preventing joint stiffness and fixed deformity of the joint.

Sr(II) and Ba(II) Alkaline Earth Metal-Organic Frameworks (AE-MOFs) for Selective Gas Adsorption, Energy Storage, and Environmental Application.

Two new alkaline earth metal-organic frameworks (AE-MOFs) containing Sr(II) (UPJS-15) or Ba(II) (UPJS-16) cations and extended tetrahedral linker (MTA) were synthesized and characterized in detail (UPJS stands for University of Pavol Jozef Safarik). Single-crystal X-ray analysis (SC-XRD) revealed that the materials are isostructural and, in their frameworks, one-dimensional channels are present with the size of ~11 × 10 Å2. The activation process of the compounds was studied by the combination of in situ heating infrared spectroscopy (IR), thermal analysis (TA) and in situ high-energy powder X-ray diffraction (HE-PXRD), which confirmed the stability of compounds after desolvation. The prepared compounds were investigated as adsorbents of different gases (Ar, N2, CO2, and H2). Nitrogen and argon adsorption measurements showed that UPJS-15 has SBET area of 1321 m2 g-1 (Ar) / 1250 m2 g-1 (N2), and UPJS-16 does not adsorb mentioned gases. From the environmental application, the materials were studied as CO2 adsorbents, and both compounds adsorb CO2 with a maximum capacity of 22.4 wt.% @ 0 °C; 14.7 wt.% @ 20 °C and 101 kPa for UPJS-15 and 11.5 wt.% @ 0°C; 8.4 wt.% @ 20 °C and 101 kPa for UPJS-16. According to IAST calculations, UPJS-16 shows high selectivity (50 for CO2/N2 10:90 mixture and 455 for CO2/N2 50:50 mixture) and can be applied as CO2 adsorbent from the atmosphere even at low pressures. The increased affinity of materials for CO2 was also studied by DFT modelling, which revealed that the primary adsorption sites are coordinatively unsaturated sites on metal ions, azo bonds, and phenyl rings within the MTA linker. Regarding energy storage, the materials were studied as hydrogen adsorbents, but the materials showed low H2 adsorption properties: 0.19 wt.% for UPJS-15 and 0.04 wt.% for UPJS-16 @ -196 °C and 101 kPa. The enhanced CO2/H2 selectivity could be used to scavenge carbon dioxide from hydrogen in WGS and DSR reactions. The second method of applying samples in the area of energy storage was the use of UPJS-15 as an additive in a lithium-sulfur battery. Cyclic performance at a cycling rate of 0.2 C showed an initial discharge capacity of 337 mAh g-1, which decreased smoothly to 235 mAh g-1 after 100 charge/discharge cycles.

Synthesis of phytoextract-mediated Ag-doped graphitic carbon nitride (Ag@GCN) for photocatalytic degradation of dyes.

The present work focuses on the green synthesis of Ag-doped graphitic carbon nitride (Ag@GCN) for photocatalytic activities, which can contribute to a more sustainable environment. The leaf extract of the Ocimum tenuiflorum (Tulsi) plant was used to prepare the silver nanoparticles, as the plant extract serves as a stabilizing and capping agent in producing silver nanoparticles. Both Ag nanoparticles and urea-derived GCN were synthesized by thermal polymerization. The Ag-doped GCN nanocomposites were synthesized using various millimolar concentrations of Ag nanoparticles (NPs) with a fixed amount of GCN. The green nanocomposites (NCs) were synthesized by calcinating leaf extract at about 550 °C. They were then characterized for surface morphology by SEM coupled with energy-dispersive X-ray spectroscopy (EDX), and elemental composition by XRD, Fourier-dispersive infrared spectroscopy (FTIR), and transmission electron microscope (TEM). Thermal stability and estimation of the Ag content in GCN were done through thermogravimetric analysis. The prepared series of nanocomposites (Ag-doped GCN 0.5 mM, 1.0 mM, 1.5 mM, 2.0 mM) were used to study the photocatalytic degradation efficiency of rose bengal (RB) and xylenol orange (XO) dyes. The degradation efficiency of dyes gets enhanced due to the doping of Ag nanoparticles into GCN. The efficiency increased from 54 to 76% and 15 to 36% in the case of RB and XO dyes, respectively. The apparent rate constant value increased up to 2.5 times in the case of the Ag-doped GCN (1.5 mM) nanocomposite in comparison to GCN. The result obtained from the study confirmed that Ag-doped GCN (1.5 mM) could act as a potential photocatalyst for wastewater remediation applications.

Visible light enhanced photocatalytic degradation of organic pollutants with SiO2/g-C3N4 nanocomposite for environmental applications.

The development of eco-friendly photocatalysts is gaining attention as an effective approach for degrading organic pollutants. In the present study, the composite materials are composed of various components with varying structures that combine to enhance their characteristics and widen their applications. This work uses the hydrothermal method for the fabrication of a novel and steady SiO2/g-C3N4 photocatalyst. The amount of SiO2 was fixed, and graphitic carbon nitride (g-C3N4) was varied in the ratio (1:x, where x = 1, 2, 3) and abbreviated as SCN1, SCN2, and SCN3. The optical properties, surface morphology, and structural analysis of the prepared nanocomposites were studied using various techniques such as FTIR, TGA, X-ray diffraction, and ultraviolet-visible spectroscopy. The results show that SCN2 nanocomposites significantly improved the photocatalytic activity, with a degradation efficiency of 70% for auramine O and 84.6% for xylenol orange dye under visible light irradiation, which is a result of their large surface area and efficient electron-hole separation rate.

Architecting the Z-scheme heterojunction of Gd2O3/g-C3N4 nanocomposites for enhanced visible-light-induced photoactivity towards organic pollutants degradation.

A basic calcination process in one step was employed to create g-C3N4 photocatalytic composites modified by Gd2O3 nanoparticles. SEM (scanning electron microscopy), FTIR (Fourier-transform infrared spectroscopy), XRD (X-ray diffraction), EIS (electrochemical impedance spectroscopy), PL (photoluminescence studies) as well as TEM (transmission electron microscopy), XPS (X-ray photoelectron spectroscopy), and CV (cyclic voltammetry) were employed to explain the structural traits, optical properties, and morphological features of the processed photocatalyst. The findings show that Gd2O3 (Gd) does not affect the sample's crystalline structure but rather increases g-C3N4 surface area by spreading it superficially. Furthermore, Gd can redshift the light absorption peak, reduce the energy gap, and improve the efficiency with which photogenerated holes and electrons are removed in g-C3N4. The surface morphology of g-C3N4, in particular, could be significantly enhanced. We similarly employed three distinct photocatalytic complexes of Gd2O3 and g-C3N4 in 1:1, 2:1, and 3:1 proportions to degrade methylene blue (MB). After 100 min in visible light (400-800 nm), the photodegradation rate of composites is 58.8% for 1:1 (GG1), 94.5% for 2:1 (GG2), and 92% for 3:1 (GG3). In addition to the MB dye, the photocatalytic activity of synthesized materials was also studied for methyl orange. The result shows phenomenal degradation values, i.e.; for GG1 86%, GG2 96%, and for GG3 84.6%. The narrow band gap that separates the photogenerated electron and hole enhances g-C3N4 ability to degrade photo-catalytically. From the result, we concluded that the photocurrent and cyclic photocatalytic degradation of methylene blue shows that a composition of 2:1 Gd2O3/g-C3N4 has high photocatalytic stability.

Mg/Li@GCN as highly active visible light responding 2D photocatalyst for wastewater remediation application.

In this study, a highly visible light responding 2D photocatalytic material has been prepared and analysed for its potential for photodegradation of organic pollutants. The pristine GCN has been co-doped with Mg/Li using the facile synthesis route. The prepared photocatalytic materials were then analysed using characterisation techniques like X-ray diffraction (XRD), Fourier-transform infrared (FTIR) spectroscopy, diffuse reflectance spectra (DRS) and photoluminescence spectroscopy (PL) analysis. The prepared samples were analysed for photocatalytic degradation analysis towards methylene blue dye. The apparent rate constant value increased up to 5.4 times in the case of the GCNML (0.5,2) sample in comparison to GCNP. In addition, the GCNML (0.5,2) sample was also analysed for degradation of crystal violet (CV) (97% in 80 min), rose bengal (RB) (84% in 120 min) and methyl orange (MO) (45% in 120 min) dyes. The result obtained from the study confirmed that GCNML (0.5,2) can act as a potential photocatalyst for wastewater remediation application.

A highly stable terbium(III) metal-organic framework MOF-76(Tb) for hydrogen storage and humidity sensing.

The present study described the synthesis and characterization of MOF-76(Tb) for hydrogen storage and humidity sensing applications. The structure and morphology of as-synthesized material were studied using powder X-ray diffraction, scanning, and transmission electron microscopy. The crystal structure of MOF-76(Tb) consists of terbium(III) and benzene-1,3,5-tricarboxylate(-III) ions, one coordinated aqua ligand and one crystallization N,N´-dimethylformamide molecule. The polymeric framework of MOF-76(Tb) contains 1D sinusoidally shaped channels with sizes of 6.6 × 6.6 Å propagating along c crystallographic axis. The thermogravimetric analysis of the prepared material exhibited thermal stability up to 600 °C. At 77 K and pressure up to 20 bar; 0.6 wt.% hydrogen storage capacity for MOF-76(Tb) was observed. Finally, the humidity sensing measurements (water adsorption experiments) were performed, and the results indicate that MOF-76(Tb) is not a suitable material for moisture sensing applications.

Tinospora cordifolia (Willd.) Hook.f. & Thomson polysaccharides: A review on extraction, characterization, and bioactivities.

Human awareness of the need for health and wellness practices that enhance disease resilience has increased as a result of recent health risks. Plant-derived polysaccharides with biological activity are good candidates to fight diseases because of their low toxicity. Tinospora cordifolia (Willd.) Hook.f. & Thomson polysaccharides extract from different plant parts have been reported to possess significant biological activity such as anti-oxidant, anti-cancer, immunomodulatory, anti-diabetic, radioprotective and hepatoprotective. Several extraction and purification techniques have been used to isolate and characterize T. cordifolia polysaccharides. Along with hot-water extraction (HWE), other novel techniques like microwave-assisted extraction (MAE), ultrasound-assisted extraction (UAE), pulsed electric field (PEF), supercritical-fluid extraction (SFE), and enzyme-assisted extraction (EAE) are used to extract T cordifolia polysaccharides. SFE is a revolutionary technology that gives the best yield and purity of low-molecular-weight polysaccharides. According to the findings, polysaccharides extracted and purified from T. cordifolia have a significant impact on their structure and biological activity. As a result, the methods of extraction, structural characterization, and biological activity of T. cordifolia polysaccharides are covered in this review. Research on T. cordifolia polysaccharides and their potential applications will benefit greatly from the findings presented in this review.

Photocatalytic dye degradation and antibacterial activities of CeO2/g-C3N4 nanomaterials for environmental applications.

The uncontrolled dumping of synthetic dyes into water sources has posed severe hazards to the ecosystem. For decades, several materials with low cost and high efficiency have been investigated for dye degradation. Photocatalytic degradation is regarded as a successful strategy since it utilizes sunlight to transform harmful pollutants into nontoxic compounds without using oxidative agents. The photocatalytic potentials of CeO2/g-C3N4 (CG) were investigated in this work using a simplistic ultrasonication process. Here, the amount of CeO2 was fixed, and g-C3N4 was varied in the ratio (1:x, where x = 1, 2, and 3) and abbreviated as CG1, CG2, and CG3. Characterization techniques such as Fourier transforms-infrared spectroscopy, thermal gravimetric analysis (TGA), powdered X-ray diffraction, ultraviolet-visible spectroscopy, etc. were used to characterize structural analysis, optical properties, particle size, and chemical bonds of the prepared nanocomposites. The photocatalytic results showed that CG2 effectively degraded rose bengal (RB) and crystal violet (CV) dyes when exposed to visible light irradiation as compared to pure GCN and CeO2. The antibacterial activity analysis further supported the potential application of prepared photocatalyst as a disinfectant agent against both gram-positive (Staphylococcus aureus and Bacillus cereus) and gram-negative (Salmonella abony and Escherichia coli) pathogenic strains of bacteria.